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| Rolland, Steven; Mengue, Luce; Noël, Cyril; Crapart, Stéphanie; Mercier, Anne; Aucher, Willy; Héchard, Yann; Samba-louaka, Ascel. |
| Acanthamoeba castellanii is a ubiquitous free-living amoeba. Pathogenic strains are causative agents of Acanthamoeba keratitis and granulomatous amoebic encephalitis. In response to adverse conditions, A. castellanii differentiate into cysts, which are metabolically inactive and resistant cells. This process, also named encystment, involves biochemical and genetic modifications that remain largely unknown. This study characterizes the role of the ACA1_384820 Acanthamoeba gene during encystment. This gene encodes a putative N-acetyltransferase, belonging to the Gcn5-related N-acetyltransferase (GNAT) family. We showed that expression of the ACA1_384820 gene was down-regulated as early as two hours after induction of encystment in A. castellanii.... |
| Tipo: Text |
Palavras-chave: Acanthamoeba castellanii; Encystment; N-acetyltransferase; Lateral gene transfer; Freeliving amoebae. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00625/73693/73214.pdf |
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| Robino, Etienne; Poirier, Aurore C.; Amraoui, Hajar; Le Bissonnais, Sandra; Perret, Angelique; Lopez‐joven, Carmen; Auguet, Jean-christophe; Rubio, Tristan P.; Cazevieille, Chantal; Rolland, Jean-luc; Héchard, Yann; Destoumieux‐garzón, Delphine; Charrière, Guillaume. |
| ibrios are ubiquitous in marine environments and opportunistically colonize a broad range of hosts. Strains of Vibrio tasmaniensis present in oyster farms can thrive in oysters during juvenile mortality events and behave as facultative intracellular pathogen of oyster hemocytes. Herein, we wondered whether V. tasmaniensis LGP32 resistance to phagocytosis is specific to oyster immune cells or contributes to resistance to other phagocytes, like marine amoebae. To address this question, we developed an integrative study, from the first description of amoeba diversity in oyster‐farms to the characterization of LGP32 interactions with amoebae. An isolate of the Vannella genus, Vannella sp. AP1411, which was collected from oyster farms, is ubiquitous, and... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00509/62109/66292.pdf |
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